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Glutamic acid decarboxylase autoantibody ELISA kit ELISA

The GADAB-ELISA kit is an accurate enzymatic assay used to measure GAD-Ab 65 in human serum to differentiate for type I diabetes mellitus.

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The GADAB-ELISA kit is an accurate enzymatic assay used to measure GAD-Ab 65 in human serum to differentiate for type I diabetes mellitus.

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Overview

Use as serological markers of type 1 diabetes mellitus (insulin-dependent diabetes mellitus - IDDM)

Benefits

  • ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

GAD65 molecules are coated onto the ELISA plate wells. GAD65-biotin complexes constitute the liquid phase. GAD-Ab from the samples bind to the GAD65immobilized onto the well and interact with GAD65-biotin via a GAD-Ab bridge. Addition of streptavidin peroxidase detects the bound GAD65-biotin. The unbound streptavidin peroxidase in excess is washed off before the addition of the peroxidase substrate TMB.

Assay procedure: 1h at RT / shaking + 1h at RT / shaking + 20 min at RT / shaking + 20 min at RT in the dark.

Features

Working range: 5 – 2000 U/mL (NIBSC 97/550)

Sample: serum – 25 µL

Analytical sensitivity: 0.57 U/mL

Normal values

Cut-offGAD-Ab U/mL
Negative<5 U/ml
Positive

>=5 U/ml

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Product Insert (ENG) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.033 - Product Insert

Product Insert (FRA) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.033 - Product Insert

Product Insert (ENG) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.037 - Product Insert

Product Insert (FRA) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.037 - Product Insert

Product Insert (ENG) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.037 - Product Insert

Product Insert (FRA) GAD / GADAB-ELISA

GADAB-ELISA Op.Mod.037 - Product Insert

Safety Data Sheet (ENG-GB) GAD / GADAB-ELISA

GADAB-ELISA - Safety Data Sheet

Quality Control Report GAD / GADAB-ELISA

GADAB-ELISA Batch KGE431 - Quality Control Report

Quality Control Report GAD / GADAB-ELISA

GADAB-ELISA Batch KGE448 - Quality Control Report